The PCR was performed utilizing Ex-Taq polymerase (TaKaRa, Shiga, Japan) via the next disorders: ninety four , sixty sec; sixty , 60 sec; 72 , 60 sec for 35 cycles. For the amplification of equally the Ex4a(+) and important WT1 [17AA(+) and 17AA(-) WT1] isoforms, primer set of Ex4-F and Ex6-R was employed. For the amplification of Ex4a(+) WT1 isoform, primer pair of 4a-F and Ex6-R was employed.The
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